Icon for: Teresa Wang

TERESA WANG

Boston University
Years in Grad School: 2

Judges’ Queries and Presenter’s Replies

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Presentation Discussion
  • Icon for: Teresa Wang

    Teresa Wang

    Presenter
    May 21, 2012 | 04:51 p.m.

    Hi everyone! Thanks for visiting this entry. My team and I are excited to share our research findings with you. Don’t hesitate to ask questions about this project, or about bioinformatics in general!

  • Icon for: Joseph Gerrein

    Joseph Gerrein

    Trainee
    May 22, 2012 | 11:21 a.m.

    Great presentation! How did you pick out differentially expressed genes using the time course data, as in what kind of statistical test did you use? Also, how did you do the pathway enrichment?

    Thanks,

    Joe

  • Icon for: Teresa Wang

    Teresa Wang

    Presenter
    May 23, 2012 | 03:08 p.m.

    Hi Joe! Thanks for your interest. We analyzed the custom-array time course data using several different techniques, one of which incorporates a linear model to adjust for changes in gene expression with time. One major challenge analysis for this project was that most non-human primates were not consistently sampled over time (sometimes due to complications with drawing blood, other times because they were more susceptible to the lethal dose of infection than others). Pathway enrichment was investigated using the Thus, aside from using a statistical test to filter for genes, we created a filter such that each probe had to have a fold change of plus/minus three in at least two arrays relative to baseline expression levels.
    For pathway enrichment, we integrated our results with tools offered by the Database for Annotation, Visualization and Integrated Discovery (DAVID) and Kyoto Encyclopedia of Genes and Genomes (KEGG).

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